Environmental DNA (eDNA) analysis enables the detection of species by identifying genetic material shed into the environment. This approach is especially valuable for monitoring species groups that are difficult to survey using acoustic, or imaging methods. In our work, eDNA is generally applied to freshwater ecosystems and soil, where aquatic species and soil-dwelling invertebrates that are otherwise hard to observe or capture reliably. Assessment begins with targeted site sampling, where water or soil samples are collected following standardised protocols to ensure consistency and data quality. These samples are then processed through laboratory analysis to extract and sequence DNA, from which a detailed and accurate species list can be generated. This provides a sensitive snapshot of local biodiversity. By integrating eDNA results with data from our other monitoring methods, we are able to build a complete and robust picture of ecosystem health. This combined approach strengthens biodiversity metrics, supports evidence-based decision-making, and enhances long-term monitoring of habitats where traditional survey techniques have practical or technical limitations.